Once you have closed the detection chamber, do not attempt to re-open the device. Opening or mishandling the chamber from a completed reaction could lead to contamination of the work area and false positive results. If you would like to keep a record of the results, Agdia recommends keeping a photographic record.
No. AmplifyRP was designed with multiple types of end users in mind. All the reagents and disposable equipment necessary to run an AmplifyRP test comes included in a kit. Any equipment required can be purchased upfront in a starter pack.
AmplifyRP kits were also designed to limit the number of steps in the protocol. All the amplification reagents are lyophilized in one-time use tubes. The only thing the end user needs to do is rehydrate the reagents and insert their sample. Once that is complete, amplification and detection can be completed in 15 to 30 minutes depending on the format.
The control line in an Acceler8 assay is a measure of the performance of the lateral flow strip, not the nucleic acid extraction. Internal reporters or housekeeping primers are used in PCR reactions to detect the presence of host (plant) nucleic acid following nucleic acid purification. AmplifyRP technology utilizes crude plant extracts and does not require any purification technique that could lead to the loss of host DNA or RNA, so internal reporters are not used.
Several. Although PCR is a highly sensitive and specific type of DNA testing, it does require some skill and is typically restricted to laboratory environments. In most PCR assays it is first required that suspect samples undergo a DNA purification or "clean-up" step. This step requires the use of expensive purification kits and sometimes toxic chemicals. PCR also requires an important step called thermal cycling where reactions are repeatedly subjected to temperature fluctuations that facilitate the actual PCR process. To summarize, PCR requires expensive equipment, skilled technicians, and several hours to complete.
AmplifyRP provides a DNA testing platform that rivals PCR in terms of sensitivity and specificity, but it eliminates the need for expensive equipment and can be completed by users of all skill levels.... all in about 30 minutes.
The total cost for equipment is as low as $300 depending on the format
Crude extracts may be used for analysis
Total assay time is between 25 and 40 minutes, including sample preparation
The amount of sample extract that you add to a reaction is dependent on the test you are running. Refer the assay user guide. Small changes to volume added (1ul or less) do not affect test performance.
If you incubate your reaction at a temperature lower than 39°C, the reaction will occur more slowly. Since a lower temperature incubation requires a longer incubation time, adding the reaction to the detection chamber too soon can lead to a false negative result.
If your heat supply has been interrupted during the reaction or if you’ve mixed your extract with the reaction pellet without immediately heating the reaction, the test may still be valid. After you have noticed that the heat supply has been interrupted or the heat block was not turned on, place your reaction back in your heat source for an additional 15 minutes and proceed with the instructions as recommended.
If a tube from a completed reaction opens before it is secured in the detection chamber, please discard the test and clean the area. If you suspect you have exposed your work area to the contents of a completed reaction, wash your hands and wipe nearby surfaces with a bleach solution before performing your next test.