Agdia

| Agdia home | product catalog| accessories|testing services| order | ask a question |

Agdia product catalog

DAS ELISA for Xylella fastidiosa

Capture by: polyclonal antibody
Detection by: polyclonal antibody
Price code: C

Acronym: Xf

Xylella fastidiosa (Xf) causes such diseases as Pierce's
disease of grapes, citrus variegated chlorosis, and phony peach
disease. The bacterium, found in xylem tissue, has a large host range,
including at least 28 families of both monocotyleyledonous and
dicotyledonous plants. It is spread mainly by sharpshooter
leafhoppers, and also by spittlebugs.

Some other diseases Xf causes are alfalfa dwarf, almond leaf scorch, plum leaf scald, periwinkle wilt, and leaf scorch of elm, oak, maple, sycamore, and mulberry. Xf is also being studied as a possible cause of the newly identified disease, oleander leaf scorch, and has been reported in coffee.

This test system is based on a mixture of antibodies from three serologically distinct isolates of Xf. It detects all of a large panel of Xf isolates, including ones from almond, citrus, grape (8 isolates), elm, mulberry (2 isloates), oak (6 isolates), periwinkle, ragweed, and sycamore. This system shows no cross-reaction with any healthy tissue tested.

Although Xf has not been shown to be the causal organism of oleander leaf scorch, this ELISA test reacts positively with samples from oleander plants with the disease.

The highest populations of Xf bacteria occur in petioles of infected plants. When symptoms are evident on leaf surfaces, the bacteria responsible are most likely located in the petioles of affected leaves. We recommend that you use leaf petiole tissue for Xf testing when possible. All other parts of the plant can also be tested with this test system.

References:

Sherald, J. L. and Lei, J. D. 1991. Evaluation of a Rapid ELISA Test Kit for Detection of Xylella fastidiosa in Landscape Trees. Plant Disease 75:200-203.

Beretta, M. J. G. et al. 1996. First report of Xylella fastidiosa in coffee. Plant Disease 80:821. D-1996-050-01N.

PRODUCTS AVAILABLE - click on the product links below to view instructions and additional information

CAB 34501/0500

Coating Antibody, includes concentrated antibody and 5 ELISA plates, 500 testwells
Price (USA): $120

CAB 34501/1000

Coating Antibody, includes concentrated antibody and 10 ELISA plates
Price (USA): $210

CAB 34501/5000

Coating Antibody, includes concentrated antibody and 50 ELISA plates, 5000 testwells
Price (USA): $765

ECP 34501/0500

Conjugated Antibody, Peroxidase label, 500 testwells
Price (USA): $150

ECP 34501/1000

Conjugated Antibody, Peroxidase label, 1000 testwells
Price (USA): $240

ECP 34501/5000

Conjugated Antibody, Peroxidase label, 5000 testwells
Price (USA): $950

LNC 34501

Negative control
Price (USA): $20

LPC 34501

Positive control, lyophilized
Price (USA): $15

PSP 34501/0096

PathoScreen kit, Peroxidase label, 96 testwells
Price (USA): $165

PSP 34501/0288

PathoScreen kit, Peroxidase label, 288 testwells
Price (USA): $305

PSP 34501/0480

PathoScreen kit, Peroxidase label, 480 testwells
Price (USA): $465

SRP 34501/0096

Reagent Set, Peroxidase label, 96 testwells
Price (USA): $80

SRP 34501/0500

Reagent Set, Peroxidase label, 500 testwells
Price (USA): $240

SRP 34501/1000

Reagent Set, Peroxidase label, 1000 testwells
Price (USA): $405

SRP 34501/5000

Reagent Set, Peroxidase label, 5000 testwells
Price (USA): $1615

Agdia testing accessories

Agdia product catalog

| Agdia home | product catalog| accessories|testing services| order | ask a question |